磁致伸縮生物傳感器系統(tǒng)設計和應用

Preface
List of figures
List of tables
List of abbreviations
List Of symbols
1 Introduction
1.1 Introduction to pathogenic bacteria and food-bome illness
1.1.1 Foodbome pathogenic bacteria
1.1.2 Infectious dose and detection of bacteria
1.2 Conventional bacterial detection methods
1.2.1 Plate counting methods
1.2.2 Enzyme-linked immunosorbent assay （ELISA）
1.2.3 Polymerase chain reaction （PCR）
1.3 Biosensors
1.3.1 Optical biosensors
1.3.2 Electrochemical biosensors
1.3.3 Acoustic wave （AW） biosensors
1.3.4 Micro-cantilever （MC） based biosensors
1.3.5 Magnetostrictive micro-cantilever （MSMC）-based biosensors
1.4 Magnetostrictive particle （MSP） based biosensors
1.4.1 Resonance behavior of MSP
1.4.2 Resonance behavior of MSP in visc6us media
1.4.3 Magnetostrictive effect and materials
1.4.4 Current status ofbiosensors based on MSPs
1.5 Sensing element for biosensors--antibodies vs. phages
1.6 Research objectives
References
2 Resonance behavior of msp and influence of surrounding media.
2.1 Introduction
2.2 Characterization of the resonance behavior of an MSP
2.3 Materials and methods
2.4 Results and discussion
2.4.1 Resonance frequency of MSPs
2.4.2 The Qvalue of MSPs
2.4.3 Resonance frequency of MSP in liquid
2.4.4 Determination of surface roughness Rave
2.4.5 Factors that affect the measured surface roughness Rave
2.4.6 Effect of liquid on Q value
2.5 Conclusions
References
3 Techniques in design and production of phage/antibody immobilized
magnetostrictive biosensor for bacterial detection
3.1 Introduction
3.2 MSP preparation
3.3 Phage and antibody immobilization
3.3.1 Phage immobilization
3.3.2 Antibody immobilization
3.4 Blocking agents
3.5 Preparation of bacterial culture
3.6 Experimental setup
3.7 SEM observation
3.8 Hill plot and kinetics of binding
3.9 Results and discussion
3.9.1 Detection of S. typhimurium
3.9.2 Detection of E. coli
3.9.3 Detection of S. aureus
3.9.4 Detection of L. Monocytogenes
3.10 Conclusions
References
4 Design and simulating techniques for portable msp biosensor system
4.1 Introduction
4.2 Equivalent circuit ofmagnetostrictive/magnetoelastic resonator
4.3 Characterization of resonance behavior ofmagnetostrictive/magnetoelastic resonator
4.3.1 Indirect approach for the frequency-domain technique
4.3.2 Time-domain technique
4.4 Conclusions
References
5 Synthesis of amorphous magnetostrictive thin film/nanowires using electrochemical deposition method
5.1 Introduction
5.2 Materials preparation
5.3 Electrochemical deposition of amorphous Co-Fe-B thin films
5.4 Electrochemical deposition of Co-Fe-B nanobars/nanotubes
5.5 Results and discussion
5.6 Conclusions
References
6 Future perspectives and recommendations